Intestinal Calcium-Binding Protein in the Developing Rat Duodenum
نویسندگان
چکیده
منابع مشابه
Intestinal membrane calcium-binding protein. Vitamin D-dependent membrane component of the intestinal calcium transport mechanism.
A particulate fraction of rat intestinal mucosal homogenates, termed the "calcium-binding complex," contains three vitamin D-dependent activities: calcium binding of high affinity, calcium-dependent adenosine triphosphatase, and p-nitrophenylphosphatase. These particulate activities vary concordantly with intestinal calcium transport, suggesting that they represent membrane components of the tr...
متن کاملStructure and nucleotide sequence of the rat intestinal vitamin D-dependent calcium binding protein gene.
The vitamin D-dependent intestinal calcium binding protein (ICaBP, 9 kDa) is under transcriptional regulation by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], the hormonal active form of the vitamin. To study the mechanism of gene regulation by 1,25-(OH)2D3, we isolated the rat ICaBP gene by using a cDNA probe. Its nucleotide sequence revealed 3 exons separated by 2 introns within approximately 3 ki...
متن کاملCalcium binding fraction detected from the brush border membrane during intestinal calcium absorption in the rat.
A fraction which had calcium binding activity was detected from the brush border membrane during intestinal calcium absorption in the rat. In situ duodenal loop experiment showed that 45Ca activity in mucosal homogenate, detergent-solubilized whole particulate fraction and detergent-solubilized brush border membrane was maximal at 30 min after the radioactive calcium solution was injected in th...
متن کاملImmunocytochemical localization of the vitamin D-dependent calcium binding protein in chick duodenum
The vitamin D-dependent calcium binding protein (CaBP) of chick duodenum has been localized by immunocytochemistry and by radioimmunoassay. Light microscopically, CaBP was seen to be present in the absorptive cells of the villi while in other cell types of the villi and the crypts, including goblet cells and endocrine cells, no CaBP was seen. At the electron microscopic level, CaBP was shown to...
متن کاملThe relationship between vitamin D-stimulated calcium transport and intestinal calcium-binding protein in the chicken.
1. The rapid stimulation of intestinal Ca(2+) transport observed in vitamin D-deficient chicks after receiving 1,25-dihydroxycholecalciferol has necessitated a re-evaluation of the correlation hitherto observed between this stimulation and the induction of calcium-binding protein synthesis. By 1h after a dose of 125ng of 1,25-dihydroxycholecalciferol, Ca(2+) transport is increased. This is at l...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1982
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-198206000-00001